K lactis protein expression kit manual

In the K. lactis Protein Expression Kit manual, a PCR method for the addition of a carboxy-terminal hemagglutinin (HA) antibody epitope tag to a secreted protein is described (Seefigure "Cloning Strategy III" in the Kit's Manual). This example can be adapted for the addition of other antibody epitope tags (e.g. FLAG or c-myc) or a. The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). The K. lactis Protein Expression Kit vector (pKLAC2) contains a variant of the strong K. lactis LAC4 promoter (PLAC4-PBI) for expression of a desired gene in K. lactis. The major advantage of the PLAC4-PBI promoter is that it is transcriptionally silent while in E. coli. In contrast, the wild-type PLAC4 promoter shows background transcriptional activity in E. coli which can be K. lactis Protein Expression Kit: ES.

In the K. lactis Protein Expression Kit manual, a PCR method for the addition of a carboxy-terminal hemagglutinin (HA) antibody epitope tag to a secreted protein is described (See Cloning Strategy III). This example can be adapted for the addition of other antibody epitope tags (e.g. FLAG or c-myc) or a hexahistidine (6-His) tag by. In the K. lactis Protein Expression Kit manual, a PCR method for the addition of a carboxy-terminal hemagglutinin (HA) antibody epitope tag to a secreted protein is described (Seefigure "Cloning Strategy III" in the Kit's Manual). This example can be adapted for the addition of other antibody epitope tags (e.g. FLAG or c-myc) or a. The K. lactis Protein Expression Kit provides a method for cloning and expressing a gene of interest in the yeast Kluyveromyces lactis (Figures 1 and 2). Proteins may be expressed intracellularly or be secreted from the cell using the supplied expression vector pKLAC2.

ニュー・イングランド・バイオラボ －NEBは制限酵素、エンドヌクレアーゼ、リコンビナント酵素、PCR用試薬、発現システム、マーカー、コンピテントセル、RNA研究用. Moreover, a vector series of Saccharomyces cerevisiae genes flanked by For expression in K. lactis, the gene encoding the Cre-. What promoter drives heterologous gene expression in K. lactis? In the K. lactis Protein Expression Kit manual, a PCR method for the addition of a.